Composite

Part:BBa_K4031017:Design

Designed by: Yuichiro Ikagawa   Group: iGEM21_Gifu   (2021-10-21)


LbCpf1(Cas12a) with affinity tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 457
    Illegal BglII site found at 2569
    Illegal BglII site found at 3306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is very long and may significantly reduce the transformation efficiency. Therefore, we recommend the use of a competent cell such as NEB-10beta, which does not reduce the transformation efficiency even with long fragments.


Source

T7 bacteriophage Tobacco Etch virus Lachnospiraceae bacterium ND2006


References